Early detection of superbugs, also known as antibiotic-resistant bacteria, is critical to the control and treatment of infections. A simple and rapid test can provide more information for early treatment of bacterial infections. The detection of relevant molecules has higher sensitivity and greater specificity. Diagnostic technologies based on the CRISPR-Cas system have been rapidly developed in recent years. So how should these systems be designed to detect superbugs quickly and reliably?

Strategy for CRISPR-CAS detection superbugs

CRISPR-Cas systems can be used to detect nucleic acids and biomarkers in bacteria or viruses. For extracted and amplified pathogenic nucleic acids, the corresponding CRISPR-Cas system can recognize and cleave them. Then, quantitative detection of pathogens is achieved by fluorescence or other methods. CRISPR-Cas system can differentiate pathogen genotypes and SNPs and is a simple, time-saving, and highly specific diagnostic method compared to traditional nucleic acid detection.